Cell Culture Supplements

L-glutamine is an important amino acid supplement commonly added to mammalian cell culture media. L-glutamine serves as an auxiliary energy source, especially when cells are rapidly dividing. L-glutamine is also important in the production of purine and pyrimidine nucleotides, amino sugars, glutathione, L-glutamate, other amino acids, and plays a role in protein synthesis and glucose production.

MCE L-Alanyl-L-Glutamine (100×) contains 200 mM L-alanyl-L-glutamine and is formulated in 0.85% NaCl. It serves as a stable dipeptide substitute for L-glutamine. Compared with free L-glutamine, it exhibits superior stability and significantly reduces the accumulation of toxic ammonia during culture, thereby improving cell viability and growth performance.

MEM Non-essential Amino Acid Solution (100×) has been filtered and can be used directly. It adds nutrients and reduces the biosynthetic burden on cell in vitro. The 100 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

This product is serum-free and supports cell growth and survival. It has been associated with the growth and expression of neuroblastoma, and with post-mitotic neuronal survival and expression in primary cultures of both the peripheral nervous system (PNS) and central nervous system (CNS). This product is formulated with water-for-injection. The 5 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

MCE N-2 Serum-free Supplement (100×) can be used in conjunction with growth factors such as bFGF and EGF in serum-free neuronal cell culture mediums, DMEM and the DMEM/F12. This product is formulated with basal culture medium. The 5 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

This product is the serum-free B-27 Supplement, the most widely cited neuronal culture supplement. It is an optimized formulation designed to support both low- and high-density growth, as well as short- and long-term viability of embryonic neurons. This product is formulated with water-for-injection. The 10 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

MCE Bi-27 Serum-free Supplement (50×) can be used to support the low- or high-density growth and short- or long-term viability of embryonic. This product is formulated with basal culture medium. The 10 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

This product is serum-free and vitamin A-free.It is a customized variant of the standard Bi-27 formulation with vitamin A removed as a supplement for neuronal cell culture to support the low- or high-density growth and short- or long-term viability of embryonic. This product is formulated with water-for-injection. The 10 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

MCE Bi-27 Serum-free Supplement (50×), Vitamin A-Free can be used to support the low- or high-density growth and short- or long-term viability of embryonic. This product is formulated with basal culture medium. The 10 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

MCE Bi-27 Serum-free Supplement (50×), Insulin-Free is a customized formulation developed by removing insulin from the standard Bi-27 formulation. As a neuronal cell culture supplement, it is specifically designed to support the growth and maintenance of hippocampal neurons and other central nervous system (CNS) neurons in applications where the presence of insulin may interfere with experimental outcomes.

MCE Bi-27 Serum-free Supplement (50×), Antioxidant-Free is a customized neuronal culture supplement developed by removing antioxidants components (such as vitamins, superoxide dismutase, catalase, etc.) from the standard Bi-27 formulation. It is specifically designed for investigating the roles of reactive oxygen species (ROS) in aging, cytotoxicity, apoptosis, and chronic neurological diseases. This formulation provides a stable culture environment for mechanistic studies without the interference of exogenous antioxidants.

MCE HAT Supplement (50×) can be added to cell culture medium as a selection agent for post-fusion selection of HGPRT-myeloma hybridoma cells used for antibody production. The 10 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

MCE HT Supplement (50×) is a liquid mixture of hypoxanthine (5 mM) and thymidine (0.8 mM), and can be added to cell culture medium as an additive for the DNA salvage synthesis pathway. The 10 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

MCE ITS Supplement (100×) can be used as a cell culture supplement and added to the medium for serum-free or low-serum cell culture systems.

MCE Lipid Concentrate (Chemically Defined) is a concentrated lipid solution with a clearly defined chemical composition, which can be added to cell culture media as an additive for serum-free or low-serum cell culture systems.

MCE Recombinant Human Serum Albumin Solution (rHSA, 20%) can be widely used in various cell culture systems and is particularly suitable for maintaining the undifferentiated growth and expansion of human embryonic stem cells (hESC) and induced pluripotent stem cells (hiPSC) under both feeder-dependent and feeder-free conditions.

MCE Sodium Pyruvate Solution (100 mM) contains 100 mM (11 g/L) sodium pyruvate and is ready for direct use in cell culture applications. The recommended working concentration is 1 mM, corresponding to a 1:100 dilution prior to use.

MCE D-Glucose Solution (200 g/L) can be used as a serum-free supplement for cell culture media and is suitable for a variety of culture systems, including but not limited to DMEM, RPMI 1640, DMEM/F-12, IMDM, and MEM.

MCE MEM Vitamin Solution (100×) contains eight vitamins, including choline chloride, D-calcium pantothenate, folic acid, nicotinamide, pyridoxal hydrochloride, riboflavin, thiamine hydrochloride, and inositol. It can be used as a serum-free supplement for cell culture media to enhance cell viability and promote cell growth.

MCE Exosome-Specific 293 Cell Medium Supplement is designed as a feed supplement for use with the Exosome-Specific 293 Cell Medium (Serum-Free) (MCE Cat. No.: HY-K3113).