enzyme activity detection

Pathways Recommended:	Metabolic Enzyme/Protease

By Targets:	Angiotensin-converting Enzyme (ACE) (1) Amino Acid Derivatives (2) Biochemical Assay Reagents (4) Caspase (1) Fluorescent Dye (7)
By Research Areas:	Cancer (1) Infection (1)

Targets Recommended: Angiotensin-converting Enzyme (ACE) NEDD8-activating Enzyme E1/E2/E3 Enzyme IRE1 TET Protein

Cat. No.	Product Name	Target	Research Areas	Chemical Structure

HY-P4426A

Lys-Pro-AMC diTFA

Fluorescent Dye Others

Lys-Pro-AMC diTFA is a fluorogenic substrate for the detection and measurement of the activity of specific enzymes .
HY-112688

H-Arg-4MβNA

Others Others

H-Arg-4MβNA is a substrate for cathepsin H, used for the detection of enzyme activity in gel electrophoresis.

Lys-Pro-AMC diTFA	Fluorescent Dye	Others
Lys-Pro-AMC diTFA is a fluorogenic substrate for the detection and measurement of the activity of specific enzymes .

H-Arg-4MβNA	Others	Others
H-Arg-4MβNA is a substrate for cathepsin H, used for the detection of enzyme activity in gel electrophoresis.

HY-W011985

H-Arg-NH2 hydrochloride	Biochemical Assay Reagents	Others
H-Arg-NH2 (hydrochloride) is a nucleophilic reagent that can be used in experiments related to enzyme activity center detection .

HY-W015996

4-Nitrophenyl-N-acetyl-α-D-galactosaminide pNP-GlcNAc	Fluorescent Dye	Others
4-Nitrophenyl-N-acetyl-α-D-galactosaminide (pNP-GlcNAc) can be used as a chromogenic substrate for N-acetyl-D-galactosaminase. 4-Nitrophenyl-N-acetyl-α-D-galactosaminide is cleaved by N-acetyl-D-galactosaminase to generate a yellow solution. The enzyme activity is quantitatively determined by absorbance detection .

HY-P4426

Lys-Pro-AMC

Fluorescent Dye Others

Lys-Pro-AMC is a fluorogenic substrate for the detection and measurement of the activity of specific enzymes .

Lys-Pro-AMC	Fluorescent Dye	Others
Lys-Pro-AMC is a fluorogenic substrate for the detection and measurement of the activity of specific enzymes .

HY-137858

H-Gly-Arg-pNA hydrochloride Gly-Arg-4-NA hydrochloride; GR-pNA hydrochloride; GR p-itoailide hydrochloride	Fluorescent Dye	Others
H-Gly-Arg-pNA hydrochloride (GR-pNA hydrochloride) is a colorimetric substrate for thrombin. H-Gly-Arg-pNA is preferentially bound by thrombin and cleaves the Gly-Arg (GR) peptide sequence, releasing p-nitroaniline (pNA) that can be detected colorimetrically at 405 nm. H-Gly-Arg-pNA hydrochloride is used as a fluorescent dye to measure thrombin activity .

HY-D0146

Resorufin benzyl ether BzRes; 7-Benzyloxyresorufin; 7-Benzyloxyphenoxazone	Fluorescent Dye	Others
Resorufin benzyl ether (BzRes), a fluorogenic enzyme substrate, can be used to detect CYP3A4 enzyme activity. Resorufin benzyl ether modified with a recognizing moiety boronate, can be used for ONOO ^- detection via a self-immolation mechanism. Ex/Em=530-570 nm/590 nm .

HY-131618

Hippuryl-Arg Bz-Gly-Arg	Amino Acid Derivatives	Others
Hippuryl-Arg (Bz-Gly-Arg) is a substrate used for fluorescence detection and is commonly used in biochemical studies, especially in studying enzyme activity. The phenylalanine portion of Hippuryl-Arg is connected by an aromatic side chain, which allows it to be detected under ultraviolet light. Hippuryl-Arg is used to detect enzymes that recognize and cut peptide bonds containing arginine .

HY-143285

AMC-GlcNAc	Fluorescent Dye	Infection
AMC-GlcNAc (compound 1) is a potent fluorogenic probe for detection and characterization of β-hexosaminidase enzyme activity. AMC-GlcNAc enables continuous monitoring by turn-on fluorescence and fluorescence signal is constant over a wide pH range (Ex=325 nm, Em=390 nm). AMC-GlcNAc enables ratiometric fluorescence detection with high sensitivity and low background, which can be used to screen for recombinant dispersin B activity in E. coli cell lysate .

HY-P4551

Hippuryl-His-Leu-OH	Amino Acid Derivatives Angiotensin-converting Enzyme (ACE)	Others
Hippuryl-His-Leu-OH is a substrate for detecting the activity of angiotensin I converting enzyme. The His-Leu released by Hippuryl-His-Leu-OH can react with o-phtbaldialdehyde or Fluorescamine (HY-D0715) for fluorescence detection .

HY-137855

4-Methylumbelliferyl sulfate potassium	Biochemical Assay Reagents	Others
4-Methylumbelliferyl sulfate (potassium), a fluorescent substrate, is commonly used to detect sulfatase activity in biochemical and biomedical research. It consists of a sulfate group attached to a fluorescent molecule, which can be cleaved by sulfatase enzymes. Upon cleavage, 4-Methylumbelliferyl sulfate releases a highly fluorescent product that can be detected using fluorescence microscopy or spectroscopy. The use of 4-Methylumbelliferyl sulfate as a substrate for sulfatase enzymes allows accurate detection and quantification of these enzymes in a variety of biological samples.

HY-120833

Ac-Ile-Glu-Thr-Asp-pNA	Caspase	Others
Ac-Ile-Glu-Thr-Asp-pNA is a substrate for caspase-8. Caspase-8 binds to and cleaves the Ile-Glu-Thr-Asp (IETD) peptide sequence to release p-nitroalinide, which can be quantified by colorimetric detection at 405 nm as a measure of enzyme activity .

HY-114879

DDAO 1 Publications Verification	Fluorescent Dye	Cancer
DDAO is a promising near-infrared (NIR) red fluorescent probewith tunable excitation wavelength (600-650nm) and longemission wavelength(λem=656nm). DDAO can de desiged for detection of the activities of different enzymes such asβ-galactosidase,sulfatase, proteinphosphatase2A,carboxylesterase 2, humanalbumin andesterases .

HY-134429

p-Nitrophenyl α-L-arabinopyranoside	Biochemical Assay Reagents	Others
p-Nitrophenyl α-L-arabinopyranoside is a biochemical reagent. p-Nitrophenyl α-L-arabinopyranoside can be hydrolyzed by recombinant BgaA (rBgaA, isolated from E. coli BL21 (DE3) strain harboring pEBGA29). p-Nitrophenyl α-L-arabinopyranoside has potential application in enzyme activity detection .

HY-W017386

3-Methyl-2-oxovaleric acid sodium	Biochemical Assay Reagents	Others
3-Methyl-2-oxovaleric acid, sodium salt belongs to the class of carboxylic acids, consisting of a five-carbon chain with methyl, carboxyl and ketone groups. This compound is commonly used as an analytical reagent in biochemical and medical research, especially for the detection and quantification of 2-ketoacids. It can also be used as a substrate in enzyme assays to measure the activity of certain enzymes involved in amino acid metabolism. In addition, 3-Methyl-2-oxovaleric acid, sodium salt may have potential research roles in various diseases such as diabetes, cancer and cardiovascular disease.

Cat. No.	Product Name	Type

HY-114879

DDAO 1 Publications Verification	Fluorescent Dyes/Probes
DDAO is a promising near-infrared (NIR) red fluorescent probewith tunable excitation wavelength (600-650nm) and longemission wavelength(λem=656nm). DDAO can de desiged for detection of the activities of different enzymes such asβ-galactosidase,sulfatase, proteinphosphatase2A,carboxylesterase 2, humanalbumin andesterases .

HY-W015996

4-Nitrophenyl-N-acetyl-α-D-galactosaminide pNP-GlcNAc	Chromogenic Substrates
4-Nitrophenyl-N-acetyl-α-D-galactosaminide (pNP-GlcNAc) can be used as a chromogenic substrate for N-acetyl-D-galactosaminase. 4-Nitrophenyl-N-acetyl-α-D-galactosaminide is cleaved by N-acetyl-D-galactosaminase to generate a yellow solution. The enzyme activity is quantitatively determined by absorbance detection .

HY-137858

H-Gly-Arg-pNA hydrochloride Gly-Arg-4-NA hydrochloride; GR-pNA hydrochloride; GR p-itoailide hydrochloride	Dyes
H-Gly-Arg-pNA hydrochloride (GR-pNA hydrochloride) is a colorimetric substrate for thrombin. H-Gly-Arg-pNA is preferentially bound by thrombin and cleaves the Gly-Arg (GR) peptide sequence, releasing p-nitroaniline (pNA) that can be detected colorimetrically at 405 nm. H-Gly-Arg-pNA hydrochloride is used as a fluorescent dye to measure thrombin activity .

HY-D0146

Resorufin benzyl ether BzRes; 7-Benzyloxyresorufin; 7-Benzyloxyphenoxazone	Dyes
Resorufin benzyl ether (BzRes), a fluorogenic enzyme substrate, can be used to detect CYP3A4 enzyme activity. Resorufin benzyl ether modified with a recognizing moiety boronate, can be used for ONOO ^- detection via a self-immolation mechanism. Ex/Em=530-570 nm/590 nm .

HY-143285

AMC-GlcNAc	Fluorescent Dyes/Probes
AMC-GlcNAc (compound 1) is a potent fluorogenic probe for detection and characterization of β-hexosaminidase enzyme activity. AMC-GlcNAc enables continuous monitoring by turn-on fluorescence and fluorescence signal is constant over a wide pH range (Ex=325 nm, Em=390 nm). AMC-GlcNAc enables ratiometric fluorescence detection with high sensitivity and low background, which can be used to screen for recombinant dispersin B activity in E. coli cell lysate .

AMC-GlcNAc

Fluorescent Dyes/Probes

AMC-GlcNAc (compound 1) is a potent fluorogenic probe for detection and characterization of β-hexosaminidase enzyme activity. AMC-GlcNAc enables continuous monitoring by turn-on fluorescence and fluorescence signal is constant over a wide pH range (Ex=325 nm, Em=390 nm). AMC-GlcNAc enables ratiometric fluorescence detection with high sensitivity and low background, which can be used to screen for recombinant dispersin B activity in E. coli cell lysate .

Cat. No.	Product Name	Type

HY-137855

4-Methylumbelliferyl sulfate potassium	Biochemical Assay Reagents
4-Methylumbelliferyl sulfate (potassium), a fluorescent substrate, is commonly used to detect sulfatase activity in biochemical and biomedical research. It consists of a sulfate group attached to a fluorescent molecule, which can be cleaved by sulfatase enzymes. Upon cleavage, 4-Methylumbelliferyl sulfate releases a highly fluorescent product that can be detected using fluorescence microscopy or spectroscopy. The use of 4-Methylumbelliferyl sulfate as a substrate for sulfatase enzymes allows accurate detection and quantification of these enzymes in a variety of biological samples.

4-Methylumbelliferyl sulfate potassium

Biochemical Assay Reagents

4-Methylumbelliferyl sulfate (potassium), a fluorescent substrate, is commonly used to detect sulfatase activity in biochemical and biomedical research. It consists of a sulfate group attached to a fluorescent molecule, which can be cleaved by sulfatase enzymes. Upon cleavage, 4-Methylumbelliferyl sulfate releases a highly fluorescent product that can be detected using fluorescence microscopy or spectroscopy. The use of 4-Methylumbelliferyl sulfate as a substrate for sulfatase enzymes allows accurate detection and quantification of these enzymes in a variety of biological samples.

HY-W017386

3-Methyl-2-oxovaleric acid sodium	Biochemical Assay Reagents
3-Methyl-2-oxovaleric acid, sodium salt belongs to the class of carboxylic acids, consisting of a five-carbon chain with methyl, carboxyl and ketone groups. This compound is commonly used as an analytical reagent in biochemical and medical research, especially for the detection and quantification of 2-ketoacids. It can also be used as a substrate in enzyme assays to measure the activity of certain enzymes involved in amino acid metabolism. In addition, 3-Methyl-2-oxovaleric acid, sodium salt may have potential research roles in various diseases such as diabetes, cancer and cardiovascular disease.

3-Methyl-2-oxovaleric acid sodium

Biochemical Assay Reagents

3-Methyl-2-oxovaleric acid, sodium salt belongs to the class of carboxylic acids, consisting of a five-carbon chain with methyl, carboxyl and ketone groups. This compound is commonly used as an analytical reagent in biochemical and medical research, especially for the detection and quantification of 2-ketoacids. It can also be used as a substrate in enzyme assays to measure the activity of certain enzymes involved in amino acid metabolism. In addition, 3-Methyl-2-oxovaleric acid, sodium salt may have potential research roles in various diseases such as diabetes, cancer and cardiovascular disease.

HY-134429

p-Nitrophenyl α-L-arabinopyranoside	Biochemical Assay Reagents
p-Nitrophenyl α-L-arabinopyranoside is a biochemical reagent. p-Nitrophenyl α-L-arabinopyranoside can be hydrolyzed by recombinant BgaA (rBgaA, isolated from E. coli BL21 (DE3) strain harboring pEBGA29). p-Nitrophenyl α-L-arabinopyranoside has potential application in enzyme activity detection .

Cat. No.	Product Name	Target	Research Area

HY-P4426A

Lys-Pro-AMC diTFA

Fluorescent Dye Others

Lys-Pro-AMC diTFA is a fluorogenic substrate for the detection and measurement of the activity of specific enzymes .

Lys-Pro-AMC diTFA	Fluorescent Dye	Others
Lys-Pro-AMC diTFA is a fluorogenic substrate for the detection and measurement of the activity of specific enzymes .

HY-120833

Ac-Ile-Glu-Thr-Asp-pNA	Caspase	Others
Ac-Ile-Glu-Thr-Asp-pNA is a substrate for caspase-8. Caspase-8 binds to and cleaves the Ile-Glu-Thr-Asp (IETD) peptide sequence to release p-nitroalinide, which can be quantified by colorimetric detection at 405 nm as a measure of enzyme activity .

HY-P4426

Lys-Pro-AMC

Fluorescent Dye Others

Lys-Pro-AMC is a fluorogenic substrate for the detection and measurement of the activity of specific enzymes .

Lys-Pro-AMC	Fluorescent Dye	Others
Lys-Pro-AMC is a fluorogenic substrate for the detection and measurement of the activity of specific enzymes .

HY-P4551

Hippuryl-His-Leu-OH	Amino Acid Derivatives Angiotensin-converting Enzyme (ACE)	Others
Hippuryl-His-Leu-OH is a substrate for detecting the activity of angiotensin I converting enzyme. The His-Leu released by Hippuryl-His-Leu-OH can react with o-phtbaldialdehyde or Fluorescamine (HY-D0715) for fluorescence detection .

HY-P5510

Ac-Asp-Glu-Asp(EDANS)-Glu-Glu-Abu-ψ-(COO)Ala-Ser-Lys(DABCYL)-NH2 HCV NS3 protease substrate	Peptides	Others
Ac-Asp-Glu-Asp(EDANS)-Glu-Glu-Abu-ψ-(COO)Ala-Ser-Lys(DABCYL)-NH2 (HCV NS3 protease substrate) is a biological active peptide. (This peptide is a HCV protease substrate incorporating an ester bond between residues P1 and P1. Due to ready transesterification of the scissile bond to the acyl-enzyme intermediate, this substrate shows very high kcat/Km values, enabling detection of activity with subnanomolar nonstructural protein 3 (NS3 protease) concentrations. It is widely used for the continuous assay of NS3 protease activity. Substrate cleavage is proportional to the enzyme concentration with a detection limit for NS3 between 1 nM and 250 pM. Upon cleavage of this substrate, fluorescence can be monitored at Abs/Em = 355/500 nm.)

MCE Japan Authorized Agent ナミキ商事株式会社コスモ・バイオ株式会社
Salutation			Country or Region *
Applicant Name *			Organization Name *
Department *
Email Address *			Product Name *
Cat. No.			Requested quantity *
Phone Number *
Remarks

Name
Email *

	Sorry, but the email address you supplied was invalid.

Inhibitors & Agonists

Fluorescent Dye

Biochemical Assay Reagents

Peptides