1. Others
  2. Fluorescent Dye
  3. Resorufin benzyl ether

Resorufin benzyl ether  (Synonyms: BzRes; 7-Benzyloxyresorufin; 7-Benzyloxyphenoxazone)

Cat. No.: HY-D0146
Handling Instructions

Resorufin benzyl ether (BzRes), a fluorogenic enzyme substrate, can be used to detect CYP3A4 enzyme activity. Resorufin benzyl ether modified with a recognizing moiety boronate, can be used for ONOO- detection via a self-immolation mechanism. Ex/Em=530-570 nm/590 nm.

For research use only. We do not sell to patients.

Resorufin benzyl ether Chemical Structure

Resorufin benzyl ether Chemical Structure

CAS No. : 87687-02-3

Size Stock
50 mg   Get quote  
100 mg   Get quote  
250 mg   Get quote  
Synthetic products have potential research and development risk.

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Top Publications Citing Use of Products
  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Resorufin benzyl ether (BzRes), a fluorogenic enzyme substrate, can be used to detect CYP3A4 enzyme activity. Resorufin benzyl ether modified with a recognizing moiety boronate, can be used for ONOO- detection via a self-immolation mechanism. Ex/Em=530-570 nm/590 nm[1][2][3].

In Vitro

CYP3A4 activity assay[2]:
1. Preparation solution.
a. The standard stock solution containing 1 mM Resorufin benzyl ether as fluorogenic substrate. Dissolve 5 mg Resorufin benzyl ether in a mixture of 1 mL of 2%w/v Poloxamer 188 (HY-D1005A), 500 mL Dimethyl sulfoxide (HY-Y0320) and 3.5 mL Acetonitrile.
b. CYP3A4 enzyme solution should be freshly prepared. Dilute 5 mL of 1 mM enzyme stock solution with 995 mL of the buffer to obtain 5 nM enzyme solution.
2. Measurement of CYP3A4 activity
a. Conduct in a 96-well plate. Add 99 mL of buffer mixture solution, 1 mL of 1 mM Resorufin benzyl ether to each well. Adjust to the final concentration of 5 mM.
b. Transfer 100 mL of 5 nM enzyme solution prior the incubation at 37 ℃ for 30 min.
c. Perform enzyme activity measurement with fluorescence detection at excitation wavelength, lex of 570 nm and emission wavelength, lem of 590 nm.
d. Factors influences in the assay of CYP3A4 activity. Such as buffer (phosphate, Tris-HCl buffer), concentration of buffer (50-200 mM) and incubation time (0-50 min).

CYP3A4 metabolism activity assay[3]:
1.Add CYP3A4 enzyme to final concentrations of 5 pmol/well.
2.Each reaction used 50 pM substrate and 200 mM potassium phosphate buffer.
3.Incubate with BzRes for 45 minutes prior to measuring metabolite fluorescence. The excitation filter (ex) is 530 nm and the emission filter (em) is 590 nm.
4.Assign the concentration designation of 100% to the full strength extract (diluted 1:4 in the final assay volume).
5.Run in duplicate for 1:3 serial dilutions of the 100% extract. Calculate mean and standard deviation of fluorescence values.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

303.31

Formula

C19H13NO3

CAS No.
SMILES

O=C1C=CC2=NC3=C(C=C(OCC4=CC=CC=C4)C=C3)OC2=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation
References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.
  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

 

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Resorufin benzyl ether
Cat. No.:
HY-D0146
Quantity:
MCE Japan Authorized Agent: