2. Recombinant Proteins
  3. OX2R Protein, Human (D100A, L143W, C147A, N202Q, Sf9, FLAG, His)

OX2R Protein, Human (D100A, L143W, C147A, N202Q, Sf9, FLAG, His)

Cat. No.: HY-P702084A
Handling Instructions

OX2R Protein, a nonselective, high-affinity receptor, functions as a key binding site for orexin-A and orexin-B neuropeptides. Its interaction with orexin-A leads to a significant increase in cytoplasmic Ca(2+) levels, indicating its involvement in cellular responses associated with neuropeptide activation. OX2R Protein, Human (D100A, L143W, C147A, N202Q, Sf9, FLAG, His) is expressed by Sf9 insect cells , with N-Flag, C-10*His labeled tag.

For research use only. We do not sell to patients.

Protein Expression Service

Size Stock
50 μg   Get quote  
100 μg   Get quote  

* Please select Quantity before adding items.

Top Publications Citing Use of Products

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

OX2R Protein, a nonselective, high-affinity receptor, functions as a key binding site for orexin-A and orexin-B neuropeptides. Its interaction with orexin-A leads to a significant increase in cytoplasmic Ca(2+) levels, indicating its involvement in cellular responses associated with neuropeptide activation. OX2R Protein, Human (D100A, L143W, C147A, N202Q, Sf9, FLAG, His) is expressed by Sf9 insect cells , with N-Flag, C-10*His labeled tag.
Background

OX2R Protein, a nonselective, high-affinity receptor, serves as a key binding site for both orexin-A and orexin-B neuropeptides. Upon orexin-A binding, OX2R triggers a notable elevation in cytoplasmic Ca(2+) levels, implicating its role in orchestrating cellular responses associated with the activation of these neuropeptides.
Species

Human
Source

Sf9 insect cells
Tag

C-10*His;N-Flag
Accession

O43614 (D33-G257&V289-L394, D100A, L143W, C147A, N202Q)
Gene ID

3062
Synonyms
HCRTR2; Orexin receptor type 2; Ox-2-R; Ox2-R; Ox2R; Hypocretin receptor type 2
Molecular Weight

42.5 kDa
Purity

Greater than 90% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
OX2R Protein, Human (D100A, L143W, C147A, N202Q, Sf9, FLAG, His)
Cat. No.:
HY-P702084A
Quantity:
MCE Japan Authorized Agent:

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Country or Region *

Email Address *

     

Phone Number *

 

Organization Name *

Remarks

Request for HNMR Report

We have received your request and will respond to you as soon as possible.