2. Recombinant Proteins
  3. Immune Checkpoint Proteins
  4. Butyrophilins
  5. BTN1A1
  6. BTN1A1 Protein, Mouse (HEK293, His)

BTN1A1 Protein, Mouse (HEK293, His)

Cat. No.: HY-P7680
Instruction de manipulation

BTN1A1 Protein, Mouse (HEK293, His) is a recombinant mouse BTN1A1 produced in HEK293 cells, with His tag. BTN1A1 is a member of butyrophilin family.

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Description

BTN1A1 Protein, Mouse (HEK293, His) is a recombinant mouse BTN1A1 produced in HEK293 cells, with His tag. BTN1A1 is a member of butyrophilin family[1].
Background

Butyrophilins belonging to the immunoglobulin superfamily are new immune system regulators because they are present on lymphocytes, dendritic cells, monocytes, macrophages, neutrophils and eosinophils, and they exert a stimulatory and (or) inhibitory effect on them[1].
Species

Mouse
Source

HEK293
Tag

C-6*His
Accession

Q62556 (A27-W247)
Gene ID

12231  [NCBI]
Molecular Construction
N-term
BTN1A1 (A27-W247)
Accession # Q62556
6*His
C-term
Synonyms
rMuBTN1A1, His; Butyrophilin subfamily 1 member A1; BTN1A1;
AA Sequence

AAPFDVTAPQEPVLALVGSDAELTCGFSPNASSEYMELLWFRQTRSTAVLLYRDGQEQEGQQMTEYRGRATLATAGLLDGRATLLIRDVRVSDQGEYRCLFKDNDDFEEAAVYLKVAAVGSDPQISMTVQENGEMELECTSSGWYPEPQVQWRTGNREMLPSTSESKKHNEEGLFTVAVSMMIRDSSIKNMSCCIQNILLGQGKEVEISLPAPFVPRLTPWHHHHHH

Masse moléculaire

32-35 kDa
Pureté

Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Instruction de manipulation (2659 KB)
Références

BTN1A1 Protein, Mouse (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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