2. Immunology/Inflammation Apoptosis
  3. COX Apoptosis
  4. Metamizole sodium

Metamizole sodium  (Synonyms: Dipyrone)

Cat. No.: HY-B1279A Purity: ≥98.0%
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Metamizole sodium (Dipyrone) is an orally active cyclooxygenase (COX) inhibitor. Metamizole sodium can inhibit cell proliferation and promote cell apoptosis. Metamizole sodium has anti-inflammatory and antioxidant activities. Metamizole sodium is an antipyretic, analgesic and spasmolytic agent. .Metamizole sodium can be used in research to relieve a variety of pain.

For research use only. We do not sell to patients.

Metamizole sodium Chemical Structure

Metamizole sodium Chemical Structure

CAS No. : 68-89-3

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Based on 1 publication(s) in Google Scholar

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Metamizole sodium Related Antibodies

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1 Publications Citing Use of MCE Metamizole sodium

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Description

Metamizole sodium (Dipyrone) is an orally active cyclooxygenase (COX) inhibitor. Metamizole sodium can inhibit cell proliferation and promote cell apoptosis. Metamizole sodium has anti-inflammatory and antioxidant activities. Metamizole sodium is an antipyretic, analgesic and spasmolytic agent. .Metamizole sodium can be used in research to relieve a variety of pain[1][2][3][4][5].

IC50 & Target

COX-3[2]
In Vitro

Metamizole sodium (1 and 10 μM, 24 h) can inhibit osteoblast differentiation and thus reduce its ability to generate new bone mineralized extracellular matrix[2].
Metamizole sodium (1-500 μM, 48 h) can inhibit cell proliferation and promote apoptosis in pancreatic cancer cell lines PaTu 8988 t and Panc-1[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Metamizole sodium Related Antibodies

Cell Proliferation Assay[3]

Cell Line: PaTu 8988 t, Panc-1
Concentration: 1, 10, 100, 250, 500 μM
Incubation Time: 48 hours
Result: Inhibited proliferation in the PaTu 8988 t cell line with 1–500 μM and with concentrations of 1 μM, 10 μM, 100 μM, and 250 μM in the Panc-1 cell line.
Increased the apoptosis rate after 9 h.
In Vivo

Metamizole sodium (500 mg/kg, intraperitoneal injection twice a day for 7 days) can reduce the development of neuropathic pain symptoms in rats[4].
Metamizole sodium (250 or 500 mg/kg, orally) can effectively relieve pain and oxidative stress induced by scalpel incision and carrageenan in rats[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male Wistar rats (270-300 g), with peripheral neuropathic pain[4]
Dosage: 500 mg/kg
Administration: Intraperitoneal injection, twice a day, for 7 days
Result: Diminished the expression of pronociceptive interleukins (IL-1beta, IL-6, and IL-18) and chemokines (CCL2, CCL4, and CCL7) in DRG measured 7 days after sciatic nerve injury.
Animal Model: Pain model in rats [5]
Dosage: 250 or 500 mg/kg
Administration: p.o.
Result: Decreased the COX-2 gene expression at 500 mg/kg and increased the MPO gene expression. Reduced MDA levels in the carrageenan paw test and increased tGSH levels.
Clinical Trial
Molecular Weight

333.34

Formula

C13H16N3NaO4S
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=S(CN(C1=C(C)N(C)N(C2=CC=CC=C2)C1=O)C)(O[Na])=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 200 mg/mL (599.99 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.9999 mL 14.9997 mL 29.9994 mL
5 mM 0.6000 mL 2.9999 mL 5.9999 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 5 mg/mL (15.00 mM); Clear solution

    This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 5 mg/mL (15.00 mM); Clear solution

    This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.9999 mL 14.9997 mL 29.9994 mL 74.9985 mL
5 mM 0.6000 mL 2.9999 mL 5.9999 mL 14.9997 mL
10 mM 0.3000 mL 1.5000 mL 2.9999 mL 7.4999 mL
15 mM 0.2000 mL 1.0000 mL 2.0000 mL 4.9999 mL
20 mM 0.1500 mL 0.7500 mL 1.5000 mL 3.7499 mL
25 mM 0.1200 mL 0.6000 mL 1.2000 mL 2.9999 mL
30 mM 0.1000 mL 0.5000 mL 1.0000 mL 2.5000 mL
40 mM 0.0750 mL 0.3750 mL 0.7500 mL 1.8750 mL
50 mM 0.0600 mL 0.3000 mL 0.6000 mL 1.5000 mL
60 mM 0.0500 mL 0.2500 mL 0.5000 mL 1.2500 mL
80 mM 0.0375 mL 0.1875 mL 0.3750 mL 0.9375 mL
100 mM 0.0300 mL 0.1500 mL 0.3000 mL 0.7500 mL
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Metamizole sodium Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Metamizole68-89-3DipyroneCOXApoptosisCyclooxygenaseInhibitorPancreatic carcinomaProliferationNeuropathic pain rat modelinhibitorinhibit

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