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  3. Mag-indo-1/AM

Mag-indo-1/AM is a cell-permeable fluorescent indicator for Mg2+ and also for Ca2+ (Ex=340-390 nm, Em=410-490 nm). Mag-indo-1/AM can be used to determine intracellular Mg2+ and Ca2+ concentrations.

For research use only. We do not sell to patients.

Mag-indo-1/AM Chemical Structure

Mag-indo-1/AM Chemical Structure

CAS No. : 130926-94-2

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Description

Mag-indo-1/AM is a cell-permeable fluorescent indicator for Mg2+ and also for Ca2+ (Ex=340-390 nm, Em=410-490 nm). Mag-indo-1/AM can be used to determine intracellular Mg2+ and Ca2+ concentrations[1][2].

In Vitro

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Measurement of intracellular Ca2+[1]:
1. Incubate 3 mL cell suspension with 10 μM Mag-indo-1/AM for 15 min at 30℃.
2. Centrifuge the cells for 1 min at 500 rpm and resuspend in 5 mL isolation buffer (containing 1 mM CaCl2).
3. Add 1 mM probenecid to the cell suspension to prevent dye leakage from the cytosol and store the cells (Mag-indo-1/AM-loaded cells) at room temperature until use.
4. Incubate the Mag-indo-1/AM-loaded cells at room temperature for 2 h, shake gently at 37℃ for 1.5 h, centrifuge the pellet, resuspend and store at room temperature.
6. Place a small portion of the loaded cells in an experimental chamber which is mounted on the stage of an inverted microscope.
7. Measure fluorescence of Mag-indo-1/AM (Ex=340-390 nm, Em=410 and 490 nm (the peak emissions of the Ca2+ bound and Ca2+ free forms of the indicator)). Fluorescence of the whole cell is collected on line by a photon counting system.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

730.63

Formula

C33H34N2O17

CAS No.
Emission (Em)

490

Excitation (Ex)

340

SMILES

O=C(C1=CC2=C(C=C1)C=C(C3=CC=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C(OCC(OCOC(C)=O)=O)=C3)N2)OCOC(C)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation
References
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Product Name:
Mag-indo-1/AM
Cat. No.:
HY-D1700
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