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  2. Fluorescent Dye Microtubule/Tubulin
  3. Flutax-2 (5/6-mixture)

Flutax-2 (5/6-mixture) is an active fluorescent derivative of paclitaxel. Flutax-2 (5/6-mixture) binds to a polymerized α,β tubulin dimer. Excitation/emission wavelength: 496/524 nm. Paclitaxel, a diterpenoid secondary metabolite produced by Taxus species, can be used for the research of a variety of cancers.

For research use only. We do not sell to patients.

Flutax-2 (5/6-mixture) Chemical Structure

Flutax-2 (5/6-mixture) Chemical Structure

Size Price Stock Quantity
100 μg USD 490 In-stock
500 μg USD 1450 In-stock
1 mg USD 2500 In-stock
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Based on 1 publication(s) in Google Scholar

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Description

Flutax-2 (5/6-mixture) is an active fluorescent derivative of paclitaxel. Flutax-2 (5/6-mixture) binds to a polymerized α,β tubulin dimer. Excitation/emission wavelength: 496/524 nm. Paclitaxel, a diterpenoid secondary metabolite produced by Taxus species, can be used for the research of a variety of cancers[1].

In Vitro

Preparation of Flutax-2 working solution
1.1 Preparation of the stock solution
Dissolve 10 mg of Flutax-2 in 1 mL of DMSO.
Note: It is recommended to store the stock solution at -20℃or -80℃away from light and avoid repetitive freeze-thaw cycles.
1.2 Preparation of Flutax-2 working solution
Dilute the stock solution in sacetate buffer to obtain 100 nM-1 μM of Flutax-2 working solution.
Note: Please adjust the concentration of Flutax-2 working solution according to the actual situation.
Cell staining
2.1 For suspension cells: Centrifuge at 1000 g at 4℃for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
For adherent cells: Discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4℃for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
2.2 Add 1 mL of Flutax-2 working solution, and then incubate at room temperature for 10-30 minutes.
2.3 Centrifuge at 400 g at 4℃for 3-4 minutes and then discard the supernatant.
2.4 Wash twice with PBS, 5 minutes each time.
2.5 Resuspend cells with serum-free cell culture medium or PBS, and then detect by fluorescence microscope or flow cytometer.
Storage
-20℃, 1 year
Protect from light

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

1319.27

Formula

C142H128F4N4O42

Appearance

Solid

Color

Light yellow to yellow

Emission (Em)

520

Excitation (Ex)

495

SMILES

O=C1OC2(C3=CC=C(C(N[C@H](C(O[C@H]([C@](C([C@H](OC(C)=O)C4=C5C)=O)(C)[C@@]6([C@H](OC(C7=CC=CC=C7)=O)[C@@](C[C@@H]5OC([C@@H]([C@@H](NC(C8=CC=CC=C8)=O)C9=CC=CC=C9)O)=O)(O)C4(C)C)[H])C[C@@]%10([H])OC[C@]%106OC(C)=O)=O)C)=O)C=C31)C%11=CC(F)=C(O)C=C%11OC%12=CC(O)=C(F)C=C2%12.O=C%13OC%14(C%15=CC(C(N[C@H](C(O[C@H]([C@](C([C@H](OC(C)=O)C%16=C%17C)=O)(C)[C@@]%18([C@H](OC(C%19=CC=CC=C%19)=O)[C@@](C[C@@H]%17OC([C@@H]([C@@H](NC(C%20=CC=CC=C%20)=O)C%21=CC=CC=C%21)O)=O)(O)C%16(C)C)[H])C[C@@]%22([H])OC[C@]%22%18OC(C)=O)=O)C)=O)=CC=C%15%13)C%23=CC(F)=C(O)C=C%23OC%24=CC(O)=C(F)C=C%14%24

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 62.5 mg/mL (47.37 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.7580 mL 3.7900 mL 7.5799 mL
5 mM 0.1516 mL 0.7580 mL 1.5160 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

=
Concentration (final)

C2

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Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 0.7580 mL 3.7900 mL 7.5799 mL 18.9499 mL
5 mM 0.1516 mL 0.7580 mL 1.5160 mL 3.7900 mL
10 mM 0.0758 mL 0.3790 mL 0.7580 mL 1.8950 mL
15 mM 0.0505 mL 0.2527 mL 0.5053 mL 1.2633 mL
20 mM 0.0379 mL 0.1895 mL 0.3790 mL 0.9475 mL
25 mM 0.0303 mL 0.1516 mL 0.3032 mL 0.7580 mL
30 mM 0.0253 mL 0.1263 mL 0.2527 mL 0.6317 mL
40 mM 0.0189 mL 0.0947 mL 0.1895 mL 0.4737 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Flutax-2 (5/6-mixture)
Cat. No.:
HY-131010
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