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  3. Fluorescein-5-thiosemicarbazide

Fluorescein-5-thiosemicarbazide 

Cat. No.: HY-D0099 Purity: 97.01%
COA Handling Instructions

Fluorescein-5-thiosemicarbazide is a amine containing fluorescent probe that can be used for labeling saccharides and protein carbonyl derivatives.

For research use only. We do not sell to patients.

Fluorescein-5-thiosemicarbazide Chemical Structure

Fluorescein-5-thiosemicarbazide Chemical Structure

CAS No. : 76863-28-0

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5 mg USD 50 In-stock
10 mg USD 80 In-stock
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Based on 1 publication(s) in Google Scholar

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Description

Fluorescein-5-thiosemicarbazide is a amine containing fluorescent probe that can be used for labeling saccharides and protein carbonyl derivatives[1][2].

Molecular Weight

421.43

Formula

C21H15N3O5S

CAS No.
Appearance

Solid

Color

Yellow to orange

Emission (Em)

522

Excitation (Ex)

485

SMILES

NNC(NC1=CC2=C(C3(C4=C(OC5=C3C=CC(O)=C5)C=C(O)C=C4)OC2=O)C=C1)=S

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (296.61 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.3729 mL 11.8644 mL 23.7287 mL
5 mM 0.4746 mL 2.3729 mL 4.7457 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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(per animal)

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Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.3729 mL 11.8644 mL 23.7287 mL 59.3218 mL
5 mM 0.4746 mL 2.3729 mL 4.7457 mL 11.8644 mL
10 mM 0.2373 mL 1.1864 mL 2.3729 mL 5.9322 mL
15 mM 0.1582 mL 0.7910 mL 1.5819 mL 3.9548 mL
20 mM 0.1186 mL 0.5932 mL 1.1864 mL 2.9661 mL
25 mM 0.0949 mL 0.4746 mL 0.9491 mL 2.3729 mL
30 mM 0.0791 mL 0.3955 mL 0.7910 mL 1.9774 mL
40 mM 0.0593 mL 0.2966 mL 0.5932 mL 1.4830 mL
50 mM 0.0475 mL 0.2373 mL 0.4746 mL 1.1864 mL
60 mM 0.0395 mL 0.1977 mL 0.3955 mL 0.9887 mL
80 mM 0.0297 mL 0.1483 mL 0.2966 mL 0.7415 mL
100 mM 0.0237 mL 0.1186 mL 0.2373 mL 0.5932 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Fluorescein-5-thiosemicarbazide
Cat. No.:
HY-D0099
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