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  3. Flubida-2

Flubida-2 is a cell permeable dye which can be hydrolyzed to Fubi-2 by endoesterases in cells (after hydrolysis, Ex=492 nm, Em=517 nm). Flubida-2 can be used to detect pH at a specific site in a cell organelle by directing the probe to where avidin fusion proteins are located.

For research use only. We do not sell to patients.

Flubida-2 Chemical Structure

Flubida-2 Chemical Structure

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Description

Flubida-2 is a cell permeable dye which can be hydrolyzed to Fubi-2 by endoesterases in cells (after hydrolysis, Ex=492 nm, Em=517 nm). Flubida-2 can be used to detect pH at a specific site in a cell organelle by directing the probe to where avidin fusion proteins are located[1].

In Vitro

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[1].
1. Dissolve Flubida-2 in DMSO (approximately 2 mM).
2. Mix the stock solution 1: 1 with 20% (w/v in DMSO) Pluronic F-127 (Molecular Probes), and dilute to the desired final concentration (2-4 μM) with serum-free DMEM.
3. 30 to 48 h post-transfection with either AV-KDEL or ST-AV DNA, HeLa cells are rinsed once with serum-free DMEM and loaded with 2-4 μM Flubida-2 for 3-5 h (or overnight for 10-15 h).
4. Chase the labeled cells with normal growth medium for at least 2 h, to allow excess dye-biotin to exit from the cytosol.
5. The strong avidin-biotin interaction ensures stable, specific avidin-Flubi- binding that resisted washing. Biotin starvation of the cells is not necessary before Flubida- loading, as staining was bright and stable.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

812.93

Formula

C86H96N8O20S2

Emission (Em)

517

Excitation (Ex)

492

SMILES

O=C(C1=CC=C(C2(OC3=O)C4=CC=C(OC(C)=O)C=C4OC5=CC(OC(C)=O)=CC=C52)C3=C1)N(C)CCCCCCN(C)C(CCCC[C@@]6([H])C(NC(N7)=O)C7CS6)=O.O=C(C8=CC=C(C9=C8)C(OC%109C%11=CC=C(OC(C)=O)C=C%11OC%12=CC(OC(C)=O)=CC=C%12%10)=O)N(C)CCCCCCN(C)C(CCCC[C@@]%13([H])C(NC(N%14)=O)C%14CS%13)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Flubida-2
Cat. No.:
HY-D1688
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