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  3. ER-Tracker Green

ER-Tracker dye is a derivative of BODIPY series dyes coupled with Glibenclamide (HY-15206), highly selective binding to the endoplasmic reticulum, non-toxic to cells at low concentrations, this type of dye is an environmentally sensitive probe, and formaldehyde treatment can still retain part of the fluorescence, with high fluorescence life, good extinction coefficient and other characteristics. Glibenclamide is an atp-dependent K+ channel blocker (Kir6, KATP) and CFTR Cl-channel blocker that binds in the endoplasmic reticulum. ER-Tracker is not suitable for staining cells after fixation.

For research use only. We do not sell to patients.

ER-Tracker Green Chemical Structure

ER-Tracker Green Chemical Structure

CAS No. : 730931-46-1

Size Price Stock Quantity
5 mg USD 350 In-stock
10 mg USD 580 In-stock
25 mg USD 1150 In-stock
50 mg USD 1850 In-stock
100 mg USD 2980 In-stock
200 mg   Get quote  
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This product is a controlled substance and not for sale in your territory.

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Based on 1 publication(s) in Google Scholar

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  • Purity & Documentation

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Description

ER-Tracker dye is a derivative of BODIPY series dyes coupled with Glibenclamide (HY-15206), highly selective binding to the endoplasmic reticulum, non-toxic to cells at low concentrations, this type of dye is an environmentally sensitive probe, and formaldehyde treatment can still retain part of the fluorescence, with high fluorescence life, good extinction coefficient and other characteristics. Glibenclamide is an atp-dependent K+ channel blocker (Kir6, KATP) and CFTR Cl-channel blocker that binds in the endoplasmic reticulum. ER-Tracker is not suitable for staining cells after fixation[1].

In Vitro

1. Preparation of ER-Tracker working solution
1.1 Preparation of the stock solution
Dissolve 100 ug ER-Tracker in 128 μL DMSO to obtain 1 mM of stock solution.
Note: It is recommended to store the stock solution at -20℃ or -80℃ away from light and avoid repetitive freeze-thaw cycles.
1.2 Preparation of ER-Tracker working solution
Dilute the stock solution in serum-free cell culture medium or PBS to obtain 100 nM-1 μM of? working solution.
Note: Please adjust the concentration of ER-Tracker working solution according to the actual situation.
2. Cell staining
2.1 Suspension cells (6-well plate)
a. Centrifuge at 1000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.The cell density is 1×106/mL.
b. Add 1 mL of working solution, and then incubate at room temperature for 5-30 minutes.
c. Centrifuge at 400 g at 4℃ for 3-4 minutes and then discard the supernatant.
d. Wash twice with PBS, 5 minutes each time.
e. Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry.
2.2 Adherent cells
a. Culture adherent cells on sterile coverslips.
b. Remove the coverslip from the medium and aspirate excess medium.
c. Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 5-30 minutes.
d. Wash twice with medium, 5 minutes each time. Observation by fluorescence microscopy or flow cytometry.
Note: If detection by flow cytometry, cells need to be resuspended before staining.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

783.09

Formula

C37H42BClF2N6O6S

CAS No.
Appearance

Solid

Color

Brown to reddish brown

Emission (Em)

520

Excitation (Ex)

489

SMILES

O=C(NS(=O)(C1=CC=C(CCNC(C2=C(OC)C(NC(CCC3=CC=C4C=C5C(C)=CC(C)=[N]5[B+3]([F-])([N-]43)[F-])=O)=CC(Cl)=C2)=O)C=C1)=O)NC6CCCCC6

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (63.85 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.2770 mL 6.3850 mL 12.7699 mL
5 mM 0.2554 mL 1.2770 mL 2.5540 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.2770 mL 6.3850 mL 12.7699 mL 31.9248 mL
5 mM 0.2554 mL 1.2770 mL 2.5540 mL 6.3850 mL
10 mM 0.1277 mL 0.6385 mL 1.2770 mL 3.1925 mL
15 mM 0.0851 mL 0.4257 mL 0.8513 mL 2.1283 mL
20 mM 0.0638 mL 0.3192 mL 0.6385 mL 1.5962 mL
25 mM 0.0511 mL 0.2554 mL 0.5108 mL 1.2770 mL
30 mM 0.0426 mL 0.2128 mL 0.4257 mL 1.0642 mL
40 mM 0.0319 mL 0.1596 mL 0.3192 mL 0.7981 mL
50 mM 0.0255 mL 0.1277 mL 0.2554 mL 0.6385 mL
60 mM 0.0213 mL 0.1064 mL 0.2128 mL 0.5321 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
ER-Tracker Green
Cat. No.:
HY-D1297
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