2. Cell Cycle/DNA Damage
  3. DNA Stain
  4. DFHBI-2T

DFHBI-2T is a membrane-permeable RNA aptamers-activated fluorescence probe (ex/em=500 nm/523 nm). DFHBI-2T is used to image RNA in live cells.

For research use only. We do not sell to patients.

DFHBI-2T Chemical Structure

DFHBI-2T Chemical Structure

CAS No. : 1539318-40-5

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5 mg USD 480 In-stock
10 mg USD 800 In-stock
25 mg USD 1550 In-stock
50 mg USD 2400 In-stock
100 mg USD 3600 In-stock
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Based on 1 publication(s) in Google Scholar

Other Forms of DFHBI-2T:

DFHBI-2T Related Antibodies

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Description

DFHBI-2T is a membrane-permeable RNA aptamers-activated fluorescence probe (ex/em=500 nm/523 nm). DFHBI-2T is used to image RNA in live cells[1][2].

In Vitro

Spinach2 imaged DFHBI-2T results in fluorescence that is detectable using the yellow emission channel[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

DFHBI-2T Related Antibodies
Molecular Weight

306.19

Formula

C12H7F5N2O2
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C1N(C)C(C(F)(F)F)=N/C1=C\C2=CC(F)=C(O)C(F)=C2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (326.59 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.2659 mL 16.3297 mL 32.6595 mL
5 mM 0.6532 mL 3.2659 mL 6.5319 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

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g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: ≥98.0%

COA (251 KB) HNMR (118 KB) LCMS (272 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.2659 mL 16.3297 mL 32.6595 mL 81.6486 mL
5 mM 0.6532 mL 3.2659 mL 6.5319 mL 16.3297 mL
10 mM 0.3266 mL 1.6330 mL 3.2659 mL 8.1649 mL
15 mM 0.2177 mL 1.0886 mL 2.1773 mL 5.4432 mL
20 mM 0.1633 mL 0.8165 mL 1.6330 mL 4.0824 mL
25 mM 0.1306 mL 0.6532 mL 1.3064 mL 3.2659 mL
30 mM 0.1089 mL 0.5443 mL 1.0886 mL 2.7216 mL
40 mM 0.0816 mL 0.4082 mL 0.8165 mL 2.0412 mL
50 mM 0.0653 mL 0.3266 mL 0.6532 mL 1.6330 mL
60 mM 0.0544 mL 0.2722 mL 0.5443 mL 1.3608 mL
80 mM 0.0408 mL 0.2041 mL 0.4082 mL 1.0206 mL
100 mM 0.0327 mL 0.1633 mL 0.3266 mL 0.8165 mL
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DFHBI-2T Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

DFHBI-2T1539318-40-5DNA StainRNAaptamersSpinach2Inhibitorinhibitorinhibit

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