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  3. BODIPY FL SSE

BODIPY FL SSE is a potent fluorescent dye. BODIPY FL SSE is used to label the primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. BODIPY FL SSE can reactive with primary amines on biomolecules to emit green fluorescence. (λex=502 nm, λem=511 nm).

For research use only. We do not sell to patients.

BODIPY FL SSE Chemical Structure

BODIPY FL SSE Chemical Structure

CAS No. : 217190-17-5

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Description

BODIPY FL SSE is a potent fluorescent dye. BODIPY FL SSE is used to label the primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. BODIPY FL SSE can reactive with primary amines on biomolecules to emit green fluorescence. (λex=502 nm, λem=511 nm)[1][2].

In Vitro

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
BODIPY FL SSE assay (to quantify GAPDH)[1]:
1.Make a 2 mg/ mL stock of GAPDH (MW 37 kDa) in PBS (pH 7.4).
2.Add 54 nM of BODIPY-FL-SSE to 1 mL of the 2 mg/ mL (54 μM) GAPDH solution. Incubate the mixture for 4 h on ice.
3.Dialyze the reaction mixture against 500 ml of PBS, pH 7.4, in a 10,000 MW-cutoff dialysis cassette for 1 h at 4 °C. Change the buffer and dialyze again overnight. Change the dialysis buffer once more the following morning and dialyze further for 1 h.
4.Run the dialyzed sample through a Zeba desalting column at 1000 g for 2 min.
5.Analyze the BD-GAPDH spectrophotometrically in a quartz cuvette. Set the wavelength scan to 250-700 nm. Blank the instrument against PBS and add the BD-GAPDH to the cuvette.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

491.21

Formula

C18H17BF2N3NaO7S

CAS No.
Emission (Em)

512

Excitation (Ex)

501

SMILES

O=C(ON(C1=O)C(C(S(=O)(O[Na])=O)C1)=O)CCC2=CC=C(C=C(C(C)=C3)[N]4=C3C)[N-]2[B+3]4([F-])[F-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation
References
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
BODIPY FL SSE
Cat. No.:
HY-D1607
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