1. Cell Cycle/DNA Damage
  2. DNA Stain
  3. Biotin-aniline

Biotin-aniline is a probe with substantially high reactivity towards RNA and DNA. Biotin-aniline emerges as more efficient probe for capturing subcellular transcriptome in living cells with high spatial specificity.

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Biotin-aniline Chemical Structure

Biotin-aniline Chemical Structure

CAS No. : 769933-15-5

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Description

Biotin-aniline is a probe with substantially high reactivity towards RNA and DNA. Biotin-aniline emerges as more efficient probe for capturing subcellular transcriptome in living cells with high spatial specificity[1].

Masse moléculaire

362.49

Formule

C18H26N4O2S

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

NC(C=C1)=CC=C1CCNC(CCCC[C@H]2[C@]3([H])[C@](NC(N3)=O)([H])CS2)=O

Livraison

Room temperature in continental US; may vary elsewhere.

Stockage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvant et solubilité
In Vitro: 

DMSO : 250 mg/mL (689.67 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7587 mL 13.7935 mL 27.5870 mL
5 mM 0.5517 mL 2.7587 mL 5.5174 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Calculateur de molarité

  • Calculateur de dilution

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Pureté et documentation
Références

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.7587 mL 13.7935 mL 27.5870 mL 68.9674 mL
5 mM 0.5517 mL 2.7587 mL 5.5174 mL 13.7935 mL
10 mM 0.2759 mL 1.3793 mL 2.7587 mL 6.8967 mL
15 mM 0.1839 mL 0.9196 mL 1.8391 mL 4.5978 mL
20 mM 0.1379 mL 0.6897 mL 1.3793 mL 3.4484 mL
25 mM 0.1103 mL 0.5517 mL 1.1035 mL 2.7587 mL
30 mM 0.0920 mL 0.4598 mL 0.9196 mL 2.2989 mL
40 mM 0.0690 mL 0.3448 mL 0.6897 mL 1.7242 mL
50 mM 0.0552 mL 0.2759 mL 0.5517 mL 1.3793 mL
60 mM 0.0460 mL 0.2299 mL 0.4598 mL 1.1495 mL
80 mM 0.0345 mL 0.1724 mL 0.3448 mL 0.8621 mL
100 mM 0.0276 mL 0.1379 mL 0.2759 mL 0.6897 mL
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Biotin-aniline Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Biotin-aniline
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