2. Cell Cycle/DNA Damage Stem Cell/Wnt JAK/STAT Signaling
  3. CDK STAT
  4. Vanicoside B

Vanicoside B is a phenylpropanoyl sucrose derivative, can be isolated from the herb Persicaria dissitiflora. Vanicoside B targets cyclin-dependent kinase 8 (CDK8) and exhibits anti-tumor activity. The potential mechanism is Vanicoside B blocks CDK8-mediated signaling pathways and decreases the expression of epithelial-mesenchymal transition proteins, so that it leads to cell cycle arrest and apoptosis.

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Vanicoside B Chemical Structure

Vanicoside B Chemical Structure

CAS No. : 155179-21-8

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Based on 1 publication(s) in Google Scholar

Vanicoside B Related Antibodies

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Description

Vanicoside B is a phenylpropanoyl sucrose derivative, can be isolated from the herb Persicaria dissitiflora. Vanicoside B targets cyclin-dependent kinase 8 (CDK8) and exhibits anti-tumor activity. The potential mechanism is Vanicoside B blocks CDK8-mediated signaling pathways and decreases the expression of epithelial-mesenchymal transition proteins, so that it leads to cell cycle arrest and apoptosis[1][2].

IC50 & Target[1]

CDK3

 

STAT3

 

In Vitro

Vanicoside B (2.5-20 μM; 72 h) shows antiproliferative activity against a panel of cancer cell lines in triple-negative breast cancer (TNBC) MDA-MB-231 cells and HCC38 cells[1].
Vanicoside B (2.5-20 μM; 72 h, 14 d, and 72 h, respectively) inhibits cell viability, colony formation, and disturbs cell cycle distribution in TNBC cells[1].
Vanicoside B (2.5-10 μM; 48 h) decreased p-STAT1, p-STAT3, and p-S6 protein level, and induces apoptosis by regulating the Skp2-p27 axis in TNBC cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Vanicoside B Related Antibodies

Western Blot Analysis[1]

Cell Line: MDA-MB-231 cells and HCC38 cells
Concentration: 0, 2.5, 5, 10 μM
Incubation Time: 48 hours
Result: Increased cleaved PARP, and p27 protein expressions, but decreased Skp2 protein level.
Suppressed CDK8 target genes and the expression of EMT-associated proteins.
Suppressed the expression of the cell proliferation marker Ki-67 in tumor tissues, also significantly suppressed the expressions of p-STAT1 (S727) and AXL.

Cell Cycle Analysis[1]

Cell Line: MDA-MB-231 cells and HCC38 cells
Concentration: 0, 2.5, 5, 10 μM
Incubation Time: 72 hours
Result: Inhibited cell cycle at sub-G1 phase.
In Vivo

Vanicoside B (5 mg/kg and 20 mg/kg; i.p.; 3 times per week for 4 weeks) inhibits tumor growth in xenografted mouse models with MDAMB-231 cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: MDA-MB-231 cell-implanted xenograft mouse model[1]
Dosage: 5 mg/kg, 20 mg/kg
Administration: Intraperitoneal injection; 3 times per week over 4 weeks
Result: Significantly reduced tumor volumes at 5 mg/kg and 20 mg/kg by 53.85% and 65.72%, respectively.
Molecular Weight

956.89

Formula

C49H48O20
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

OC(C=C1)=CC=C1/C=C/C(OC[C@@]2([C@H]([C@@H]([C@H](O2)COC(/C=C/C3=CC=C(C=C3)O)=O)O)OC(/C=C/C4=CC=C(C=C4)O)=O)O[C@H]5O[C@@H]([C@H]([C@@H]([C@H]5O)O)O)COC(/C=C/C6=CC(OC)=C(C=C6)O)=O)=O
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (104.51 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.0451 mL 5.2253 mL 10.4505 mL
5 mM 0.2090 mL 1.0451 mL 2.0901 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (2.61 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (2.61 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
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%
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Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.0451 mL 5.2253 mL 10.4505 mL 26.1263 mL
5 mM 0.2090 mL 1.0451 mL 2.0901 mL 5.2253 mL
10 mM 0.1045 mL 0.5225 mL 1.0451 mL 2.6126 mL
15 mM 0.0697 mL 0.3484 mL 0.6967 mL 1.7418 mL
20 mM 0.0523 mL 0.2613 mL 0.5225 mL 1.3063 mL
25 mM 0.0418 mL 0.2090 mL 0.4180 mL 1.0451 mL
30 mM 0.0348 mL 0.1742 mL 0.3484 mL 0.8709 mL
40 mM 0.0261 mL 0.1306 mL 0.2613 mL 0.6532 mL
50 mM 0.0209 mL 0.1045 mL 0.2090 mL 0.5225 mL
60 mM 0.0174 mL 0.0871 mL 0.1742 mL 0.4354 mL
80 mM 0.0131 mL 0.0653 mL 0.1306 mL 0.3266 mL
100 mM 0.0105 mL 0.0523 mL 0.1045 mL 0.2613 mL
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Vanicoside B Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Vanicoside B155179-21-8CDKSTATCyclin dependent kinaseInhibitorinhibitorinhibit

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