Notch pathway assay is an experimental method used to analyze and identify the activity of the Notch signaling pathway. The Notch pathway plays a key role in processes such as cell differentiation, proliferation, and apoptosis. In order to detect Notch pathway activity, commonly used methods include real-time fluorescence quantitative PCR (qPCR) to detect Notch pathway-related gene expression, Western blot to analyze the translation level of Notch pathway proteins, and immunohistochemistry or immunofluorescence staining to detect Notch pathway proteins in cells or tissues. expression and positioning.