2. Immunology/Inflammation
  3. PD-1/PD-L1
  4. PD-L1-IN-3

PD-L1-IN-3 (Compound 4a) is a compound that targets PD-1/PD-L1, the IC50 value and EC50 value is 4.97nM and 2.70 μM for inhibit PD-L1 and Jurkat T cells, respectively. PD-L1-IN-3 can bind PD-L1 dimer to prevent PD-1 binding to PD-L1, therefore blocking PD-1 signaling. PD-L1-IN-3 can be used for lung cancer and melanoma diseases research.

For research use only. We do not sell to patients.

PD-L1-IN-3 Chemical Structure

PD-L1-IN-3 Chemical Structure

CAS No. : 2953044-29-4

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Based on 1 publication(s) in Google Scholar

PD-L1-IN-3 Related Antibodies

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Description

PD-L1-IN-3 (Compound 4a) is a compound that targets PD-1/PD-L1, the IC50 value and EC50 value is 4.97nM and 2.70 μM for inhibit PD-L1 and Jurkat T cells, respectively. PD-L1-IN-3 can bind PD-L1 dimer to prevent PD-1 binding to PD-L1, therefore blocking PD-1 signaling. PD-L1-IN-3 can be used for lung cancer and melanoma diseases research[1].

In Vitro

PD-L1-IN-3 (Compound 4a) (0.01-100 μM, 40 min) disrupts the binding between PD-1 and PD-L1 and enhances a TCR-mediated activation of the Jurkat cells[1].
PD-L1-IN-3 (0.01-100 μM, 40 min) has higher uptake which correlated with PD-L1 expression in PD-L1+ H358 tumors[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

PD-L1-IN-3 Related Antibodies

Cell Viability Assay[1]

Cell Line: Jurkat cells
Concentration: 0.01-100 μM
Incubation Time: 40 min
Result: Observed EC50 value of 2.70 μM.

Immunofluorescence[1]

Cell Line: PD-L1+/− (H358 and ES2) tumor
Concentration: 0.01-100 μM
Incubation Time: 40 min
Result: Observed 40-55% higher uptake in PD-L1+ H358 tumors than in wild-type counterparts.
Failed to discriminate between wild-type and knock-out for ES2 slides.
In Vivo
Animal Model: PD-L1+/− (H358 and ES2) tumor[1]
Dosage: 0.01-100 μM
Administration: incubation
Result: Observed 40-55% higher uptake in PD-L1+ H358 tumors than in wild-type counterparts.
Failed to discriminate between wild-type and knock-out for ES2 slides.
Molecular Weight

374.86

Formula

C19H16ClFN2OS
CAS No.
Unlabeled Cas

Appearance

Solid

Color

White to off-white

SMILES

FC1=CC=C(C2=CSC(OCC3=CC=C(CN)C=C3)=C2C#N)C=C1.Cl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (333.46 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.6677 mL 13.3383 mL 26.6766 mL
5 mM 0.5335 mL 2.6677 mL 5.3353 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.6677 mL 13.3383 mL 26.6766 mL 66.6916 mL
5 mM 0.5335 mL 2.6677 mL 5.3353 mL 13.3383 mL
10 mM 0.2668 mL 1.3338 mL 2.6677 mL 6.6692 mL
15 mM 0.1778 mL 0.8892 mL 1.7784 mL 4.4461 mL
20 mM 0.1334 mL 0.6669 mL 1.3338 mL 3.3346 mL
25 mM 0.1067 mL 0.5335 mL 1.0671 mL 2.6677 mL
30 mM 0.0889 mL 0.4446 mL 0.8892 mL 2.2231 mL
40 mM 0.0667 mL 0.3335 mL 0.6669 mL 1.6673 mL
50 mM 0.0534 mL 0.2668 mL 0.5335 mL 1.3338 mL
60 mM 0.0445 mL 0.2223 mL 0.4446 mL 1.1115 mL
80 mM 0.0333 mL 0.1667 mL 0.3335 mL 0.8336 mL
100 mM 0.0267 mL 0.1334 mL 0.2668 mL 0.6669 mL
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PD-L1-IN-3 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

PD-L1-IN-32953044-29-4PD-1/PD-L1PD-1/Programmed death-ligand 1small molecularlung cancerInhibitorinhibitorinhibit

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