1. Academic Validation
  2. Octreotide attenuates intestinal barrier damage by maintaining basal autophagy in Caco2 cells

Octreotide attenuates intestinal barrier damage by maintaining basal autophagy in Caco2 cells

  • Mol Med Rep. 2024 Jun;29(6):90. doi: 10.3892/mmr.2024.13214.
Xiaoli Liu # 1 Yan Zhou # 2 Yu Zhang # 3 Xigang Cui 4 Donglin Yang 1 Yuling Li 1
Affiliations

Affiliations

  • 1 School of Basic Medical Sciences, Binzhou Medical University, Yantai Affiliated Hospital of Binzhou Medical University, Yantai, Shandong 264003, P.R. China.
  • 2 Department of Gastrointestinal Surgery, Yantai Mountain Hospital, Yantai, Shandong 264003, P.R. China.
  • 3 Department of Gastrointestinal Surgery, Yantai Affiliated Hospital of Binzhou Medical University, Yantai, Shandong 264003, P.R. China.
  • 4 Department of Gastrointestinal and Thyroid Surgery, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, Shandong 264000, P.R. China.
  • # Contributed equally.
Abstract

The intestinal mucosal barrier is of great importance for maintaining the stability of the internal environment, which is closely related to the occurrence and development of intestinal inflammation. Octreotide (OCT) has potential applicable clinical value for treating intestinal injury according to previous studies, but the underlying molecular mechanisms have remained elusive. This article is based on a cell model of inflammation induced by lipopolysaccharide (LPS), aiming to explore the effects of OCT in protecting intestinal mucosal barrier function. A Cell Counting Kit‑8 assay was used to determine cell viability and evaluate the effectiveness of OCT. Gene silencing technology was used to reveal the mediated effect of Somatostatin Receptor 2 (SSTR2). The changes in intestinal permeability were detected through trans‑epithelial electrical resistance and fluorescein isothiocyanate‑dextran 4 experiments, and the alterations in tight junction proteins were detected using immunoblotting and reverse transcription fluorescence‑quantitative PCR technology. Autophagosomes were observed by electron microscopy and the dynamic changes of the Autophagy process were characterized by LIGHT chain (LC)3‑II/LC3‑I conversion and autophagic flow. The results indicated that SSTR2‑dependent OCT can prevent the decrease in cell activity. After LPS treatment, the permeability of monolayer cells decreased and intercellular tight junctions were disrupted, resulting in a decrease in tight junction protein zona occludens 1 in cells. The level of autophagy‑related protein LC3 was altered to varying degrees at different times. These abnormal changes gradually returned to normal levels after the combined application of LPS and SSTR2‑dependent OCT, confirming the role of OCT in protecting intestinal barrier function. These experimental results suggest that OCT maintains basal Autophagy and cell activity mediated by SSTR2 in intestinal epithelial cells, thereby preventing the intestinal barrier dysfunction in inflammation injury.

Keywords

Caco2 cells; autophagy; intestinal barrier function; intestinal epithelial cells; octreotide; somatostatin receptors.

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