1. Academic Validation
  2. Berberine decreases S100B generation to regulate gut vascular barrier permeability in mice with burn injury

Berberine decreases S100B generation to regulate gut vascular barrier permeability in mice with burn injury

  • Pharm Biol. 2024 Dec;62(1):53-61. doi: 10.1080/13880209.2023.2291679.
Aiwen Feng 1 2 Shaosheng Su 1 Cheng Li 2 Yutian Kang 3 Jiasheng Qiu 1 2 Jun Zhou 1 2
Affiliations

Affiliations

  • 1 Department of General Surgery, Maoming People's Hospital, Guangdong Medical University, China.
  • 2 Department of General Surgery, Maoming People's Hospital, Southern Medical University, China.
  • 3 Department of Burn and Plastic Surgery, Maoming People's Hospital, Southern Medical University, China.
Abstract

Context: Berberine (BBR) can regulate enteric glial cells (EGCs) and the gut vascular barrier (GVB).Objective: To explore whether BBR regulates GVB permeability via the S100B pathway.Materials and methods: GVB hyperpermeability in C57BL/6J mice was induced by burns or S100B enema. BBR (25 or 50 mg/kg/d, 3 d) was gavaged preburn. S100B monoclonal antibody (S100BmAb) was i.v. injected postburn. Mouse intestinal microvascular endothelial cells (MIMECs) were treated with S100B, S100B plus BBR, or Z-IETD-FMK. GVB permeability was assayed by FITC-dextran, S100B by ELISA, Caspase-8, β-catenin, occludin and PV-1 by immunoblot.Results: Burns elevated S100B in serum and in colonic mucosa to a peak (147.00 ± 4.95 ng/mL and 160.30 ± 8.50 ng/mg, respectively) at 36 h postburn, but BBR decreased burns-induced S100B in serum (126.20 ± 6.30 or 90.60 ± 3.78 ng/mL) and in mucosa (125.80 ± 12.40 or 91.20 ± 8.54 ng/mg). Burns raised GVB permeability (serum FITC-dextran 111.40 ± 8.56 pg/mL) at 48 h postburn, but BBR reduced GVB permeability (serum FITC-dextran 89.20 ± 6.98 or 68.60 ± 5.50 ng/mL). S100B enema (1 μM) aggravated burns-raised GVB permeability (142.80 ± 8.07 pg/mL) and PV-1, but the effect of S100B was antagonized by BBR. Z-IETD-FMK (5 μM) increased S100B-induced permeability to FITC-dextran (205.80 ± 9.70 to 263.80 ± 11.04 AUs) while reducing β-catenin in MIMECs. BBR (5 μM) reduced S100B-induced permeability (104.20 ± 9.65 AUs) and increased Caspase-8, β-catenin and occludin.Discussion and conclusion: BBR decreases burns-induced GVB hyperpermeability via modulating S100B/Caspase-8/β-catenin pathway and may involve EGCs.

Keywords

Enteric glial cells; caspase-8; occludin; β-catenin.

Figures