2. Autophagy
  3. Atg8/LC3 Autophagy
  4. DC-LC3in-D5

DC-LC3in-D5 acts as an autophagy inhibitor by attenuating LC3B lipidation. DC-LC3in-D5 binds with LC3B. DC-LC3in-D5 disrupts the LC3B-LBP2 interaction with an IC50 of 200 nM. DC-LC3in-D5 may contribute to anti-HCV or combination researchs in cancer through inhibiting autophagy.

For research use only. We do not sell to patients.

DC-LC3in-D5 Chemical Structure

DC-LC3in-D5 Chemical Structure

CAS No. : 2868312-73-4

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 330 In-stock
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10 mM * 1 mL in DMSO USD 330 In-stock
Solid
5 mg USD 300 In-stock
10 mg USD 480 In-stock
25 mg USD 980 In-stock
50 mg USD 1500 In-stock
100 mg USD 2350 In-stock
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Based on 1 publication(s) in Google Scholar

DC-LC3in-D5 Related Antibodies

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Description

DC-LC3in-D5 acts as an autophagy inhibitor by attenuating LC3B lipidation. DC-LC3in-D5 binds with LC3B. DC-LC3in-D5 disrupts the LC3B-LBP2 interaction with an IC50 of 200 nM. DC-LC3in-D5 may contribute to anti-HCV or combination researchs in cancer through inhibiting autophagy[1].

In Vitro

DC-LC3in-D5 demonstrates high selectivity to LC3A/B in the proteome. DC-LC3in-D5 exhibits a potent covalent reactivity and selectivity to LC3A/B in HeLa cells[1].
? Treatment of HeLa cells with DC-LC3in-D5 (3-30 μM) results in disruption of LC3B lipidation, inhibition of autophagic vesicle formation, and accumulation of p62[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

DC-LC3in-D5 Related Antibodies

Western Blot Analysis[1]

Cell Line: HeLa cells
Concentration: 3, 10, 30 μM
Incubation Time: 16 hours
Result: Pre-treated accumulated significant more p62 than DMSO-treated control samples. Attenuated LC3-I/II lipidation in cells exposed to autophagy inducing conditions.
Molecular Weight

397.30

Formula

C19H22Cl2N2O3
CAS No.
Appearance

Solid

Color

White to light yellow

SMILES

O=C1/C(C(CC(C2=C(Cl)C(Cl)=CC=C2)C1)=O)=C/NCCN3CCOCC3
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 35.71 mg/mL (89.88 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.5170 mL 12.5849 mL 25.1699 mL
5 mM 0.5034 mL 2.5170 mL 5.0340 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.5170 mL 12.5849 mL 25.1699 mL 62.9247 mL
5 mM 0.5034 mL 2.5170 mL 5.0340 mL 12.5849 mL
10 mM 0.2517 mL 1.2585 mL 2.5170 mL 6.2925 mL
15 mM 0.1678 mL 0.8390 mL 1.6780 mL 4.1950 mL
20 mM 0.1258 mL 0.6292 mL 1.2585 mL 3.1462 mL
25 mM 0.1007 mL 0.5034 mL 1.0068 mL 2.5170 mL
30 mM 0.0839 mL 0.4195 mL 0.8390 mL 2.0975 mL
40 mM 0.0629 mL 0.3146 mL 0.6292 mL 1.5731 mL
50 mM 0.0503 mL 0.2517 mL 0.5034 mL 1.2585 mL
60 mM 0.0419 mL 0.2097 mL 0.4195 mL 1.0487 mL
80 mM 0.0315 mL 0.1573 mL 0.3146 mL 0.7866 mL
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DC-LC3in-D5 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

DC-LC3in-D52868312-73-4Atg8/LC3AutophagyAutophagy-related 8| LC3B lipidation ,LC3B-LBP2 interactionanti-HCVcancerInhibitorinhibitorinhibit

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