Acetyl-Histone H3 (Lys4) Antibody

Acetyl-Histone H3 (Lys4) Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 15 kDa, targeting to Acetyl-Histone H3 (Lys4). It can be used for WB,ICC/IF assays with tag free, in the background of Human, Mouse.

Acetyl-Histone H3 (Lys4): Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]

Free

8350

Predicted band size: 15 kDa

affinity purified

Non-conjugated

Acetylated

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse

P68431

WB: 1:500-1:1,000 ;IF: 1:50-1:200

• 
  Western blot analysis of extracts from Hela(lane 2(20μg) and Hela (lane 3(20μg)), Trichostatin A treated (HY-15144, 1μM, 18hrs) as indicated, using Acetyl-Histone H3 (Lys4) (HY-P80531) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80531, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

WB, ICC/IF

Liquid

Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to residues surrounding Lys4 of Human Human Histone H3.The exact sequence is proprietary to MCE.