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  3. Fluorescent Dye
  4. DAF-FM DA

DAF-FM DA  (Synonyms: Diaminofluorescein-FM diacetate)

Cat. No.: HY-D0717 Purity: ≥98.0%
COA Handling Instructions

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DAF-FM DA (Diaminofluorescein-FM diacetate) is a fluorescent probe for the detection and bioimaging of nitric oxide (NO). DAF-FM DA spontaneously crosses the plasma membrane and is subsequently cleaved by esterases to generate intracellular DAF-FM (Ex/Em=495/515 nm).

For research use only. We do not sell to patients.

DAF-FM DA Chemical Structure

DAF-FM DA Chemical Structure

CAS No. : 254109-22-3

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Solvent
100μg (5 mM * 40 μL in DMSO) USD 180 In-stock
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Based on 8 publication(s) in Google Scholar

DAF-FM DA Related Antibodies

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Description

DAF-FM DA (Diaminofluorescein-FM diacetate) is a fluorescent probe for the detection and bioimaging of nitric oxide (NO). DAF-FM DA spontaneously crosses the plasma membrane and is subsequently cleaved by esterases to generate intracellular DAF-FM (Ex/Em=495/515 nm)[1].

In Vitro

The BCa cells are pre-incubated with 5 μM DAF-FM DA for 30 min to load the probe. Subsequently, the cells were incubated with fresh Hank's buffer for 20 min to complete de-esterification. The DAF-FM DA probe quantifies nitric oxide (NO) levels in cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

DAF-FM DA Related Antibodies
In Vivo

Using sections of zebrafish embryos live-stained with DAF-FM DA (5 μM), could confirm that the fluorescent signals were predominantly located in areas of ongoing bone formation[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

496.42

Formula

C25H18F2N2O7
CAS No.
Unlabeled CAS

Appearance

Solid

Color

White to off-white

Emission (Em)

515
Excitation (Ex)

495
SMILES

O=C1OC2(C3=C(OC4=C2C=C(F)C(OC(C)=O)=C4)C=C(OC(C)=O)C(F)=C3)C5=C1C(N)=C(NC)C=C5
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Purity & Documentation

Purity: ≥98.0%

COA (256 KB)

Handling Instructions (2659 KB)
Dyeing Example
References
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DAF-FM DA Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

DAF-FM DA254109-22-3Diaminofluorescein-FM diacetateFluorescent DyeNOInhibitorinhibitorinhibit

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